R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CD83 (D8V7V) Rabbit mAb #99075
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 23-65 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CD83 (D8V7V) Rabbit mAb recognizes endogenous levels of total CD83 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro63 of human CD83 protein.
Background
CD83 is a single-transmembrane protein with a calculated molecular weight (MW) of 23 kDa, but due to heavy and differential glycosylation, its apparent MW ranges from 23 to 70 kDa. CD83 is predominantly expressed on mature dendritic cells (DCs) and has been used as a DC activation/maturation marker as its increased expression is correlated with upregulation of HLA class II antigen expression on DCs (1-3). CD83 is also expressed at a low level on lymphocytes and is upregulated upon lymphocyte activation (4,5). Thymic epithelial cells also express CD83, which is required for normal CD4+ T cell development (6,7). CD83 is also expressed as a soluble form (sCD83) that can be found in serum of healthy adults (8). sCD83 has been shown to negatively regulate immune response by lymphocytes (9,10).
- Zhou, L.J. et al. (1992) J Immunol 149, 735-42.
- Prechtel, A.T. and Steinkasserer, A. (2007) Arch Dermatol Res 299, 59-69.
- Prazma, C.M. and Tedder, T.F. (2008) Immunol Lett 115, 1-8.
- Prazma, C.M. et al. (2007) J Immunol 179, 4550-62.
- Lüthje, K. et al. (2008) Int Immunol 20, 949-60.
- Fujimoto, Y. et al. (2002) Cell 108, 755-67.
- Breloer, M. and Fleischer, B. (2008) Trends Immunol 29, 186-94.
- Hock, B.D. et al. (2001) Int Immunol 13, 959-67.
- Lin, W. et al. (2017) Sci Rep 7, 12895.
- Horvatinovich, J.M. et al. (2017) J Immunol 198, 2286-2301.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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