R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CD81 (E2K9V) Rabbit mAb (BSA and Azide Free) #99507
Filter:
- WB
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 22 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This product is the carrier free version of product #52892. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #52892
For standard formulation of this product see product #52892
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
CD81 (E2K9V) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD81 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to a central extracellular region of human CD81 protein.
Background
CD81 belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2) (1-3). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin enriched microdomains (TEMs) where they mediate a range of processes including adhesion, motility, membrane organization, and signal transduction (3). CD81, like other tetraspanins, is enriched in exosomes (4). Many research studies demonstrate a role for CD81 in lymphocyte signaling (5-7). CD81 is also a well-characterized receptor for Hepatitis C Virus and facilitates the entry of the virus into target cells (8, 9).
- Oren, R. et al. (1990) Mol Cell Biol 10, 4007-15.
- Levy, S. et al. (1991) J Biol Chem 266, 14597-602.
- Hemler, M.E. (2005) Nat Rev Mol Cell Biol 6, 801-11.
- Escola, J.M. et al. (1998) J Biol Chem 273, 20121-7.
- Levy, S. et al. (1998) Annu Rev Immunol 16, 89-109.
- Levy, S. and Shoham, T. (2005) Nat Rev Immunol 5, 136-48.
- Jones, E.L. et al. (2011) Biochem Soc Trans 39, 506-11.
- Pileri, P. et al. (1998) Science 282, 938-41.
- Bartosch, B. et al. (2003) J Biol Chem 278, 41624-30.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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