R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CD81 (D5O2Q) Rabbit mAb #10037
Filter:
- WB
- IP
- IHC
- IF
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 22 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:400 |
| Flow Cytometry (Live) | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #79273.
For a carrier free (BSA and azide free) version of this product see product #79273.
Protocol
Specificity / Sensitivity
CD81 (D5O2Q) Rabbit mAb recognizes endogenous levels of total CD81 protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro184 of mouse CD81 protein.
Background
CD81 belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2) (1-3). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin enriched microdomains (TEMs) where they mediate a range of processes including adhesion, motility, membrane organization, and signal transduction (3). CD81, like other tetraspanins, is enriched in exosomes (4). Many research studies demonstrate a role for CD81 in lymphocyte signaling (5-7). CD81 is also a well-characterized receptor for Hepatitis C Virus and facilitates the entry of the virus into target cells (8,9).
- Oren, R. et al. (1990) Mol Cell Biol 10, 4007-15.
- Levy, S. et al. (1991) J Biol Chem 266, 14597-602.
- Hemler, M.E. (2005) Nat Rev Mol Cell Biol 6, 801-11.
- Escola, J.M. et al. (1998) J Biol Chem 273, 20121-7.
- Levy, S. et al. (1998) Annu Rev Immunol 16, 89-109.
- Levy, S. and Shoham, T. (2005) Nat Rev Immunol 5, 136-48.
- Jones, E.L. et al. (2011) Biochem Soc Trans 39, 506-11.
- Pileri, P. et al. (1998) Science 282, 938-41.
- Bartosch, B. et al. (2003) J Biol Chem 278, 41624-30.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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