R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CD6 (E9Y7Y) Rabbit mAb #40623
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100-140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #36549.
For a carrier free (BSA and azide free) version of this product see product #36549.
Protocol
Specificity / Sensitivity
CD6 (E9Y7Y) Rabbit mAb recognizes endogenous levels of total CD6 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD6 protein.
Background
T cell differentiation antigen CD6 is a cell adhesion molecule expressed on immature thymocytes and mature T cells, and has also been detected on a subset of B cells and NK cells within the immune system (1-4). CD6 mediates cell-cell interactions through its binding partner CD166/ALCAM (2), and contributes to the formation and maturation of the immunological synapse (3,4). CD6 functions as a co-stimulatory receptor, promoting T cell activation and proliferation through the TCR/CD3 complex signaling cascade (3-6). Studies have shown CD6 can be glycosylated (7), hyperphosphorylated on serine and threonine residues (8), and phosphorylated on tyrosine residues (6,9), each of which can differentially affect the function and signaling of this molecule. CD6 also functions as a calcium-dependent pattern receptor that binds and aggregates Gram-positive and Gram-negative bacteria. In response to lipopolysaccharide, CD6 mediates activation of the inflammatory response and secretion of pro-inflammatory cytokines (10).
- Bowen, M.A. et al. (1997) J Immunol 158, 1149-56.
- Hassan, N.J. et al. (2004) Eur J Immunol 34, 930-40.
- Gimferrer, I. et al. (2004) J Immunol 173, 2262-70.
- Zimmerman, A.W. et al. (2006) Blood 107, 3212-20.
- Roncagalli, R. et al. (2014) Nat Immunol 15, 384-392.
- Hassan, N.J. et al. (2006) Mol Cell Biol 26, 6727-38.
- Escoda-Ferran, C. et al. (2014) FEBS Lett 588, 2805-13.
- Swack, J.A. et al. (1991) J Biol Chem 266, 7137-43.
- Wee, S. et al. (1993) J Exp Med 177, 219-23.
- Sarrias, M.R. et al. (2007) Proc Natl Acad Sci USA 104, 11724-9.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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