CD57 (HNK-1) Mouse mAb #72031
Filter:
- IHC
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgM |
Application Key:
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| IHC Leica Bond | 1:400 - 1:1600 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
Storage
Supplied in 10 mM PBS containing 0.05% BSA and 0.05% sodium azide. Stable for 36 months when stored at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance.
Protocol
Specificity / Sensitivity
CD57 (HNK-1) Mouse mAb recognizes the CD57 carbohydrate modification produced by the B3GAT1 protein enzyme.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a membrane antigen from HSB-2 cells.
Background
CD57 antigen, also known as HNK-1 and Leu7, is a terminally sulfated glycan carbohydrate epitope (glyco-epitope) expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans at the cell surface (1,2). CD57 is synthesized by the enzyme B3GAT1 and is present on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells, as well as neural cells and striated muscle (3-5). Studies have shown that CD57 is not expressed on platelets, monocytes, granulocytes, or red blood cells (6). The CD57 epitope may play a role in neural cell adhesion (7), and characterizes unique maturation states in T and NK cells (8,9). CD57 is an important marker when studying functional immune deficiency in patients with autoimmune diseases, infectious diseases, and cancers (1).
- Focosi, D. et al. (2010) J Leukoc Biol 87, 107-16.
- Górska, A. et al. (2016) Arch Immunol Ther Exp (Warsz) 64, 497-503.
- Kared, H. et al. (2016) Cancer Immunol Immunother 65, 441-52.
- Lopez-Vergès, S. et al. (2010) Blood 116, 3865-74.
- Mechtersheimer, G. et al. (1991) Cancer Res 51, 1300-7.
- Stelin, S. et al. (2009) J Indian Soc Periodontol 13, 150-4.
- Künemund, V. et al. (1988) J Cell Biol 106, 213-23.
- Sze, D.M. et al. (2001) Blood 98, 2817-27.
- Nielsen, C.M. et al. (2013) Front Immunol 4, 422.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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