R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CD40 Ligand (D5J9Y) Rabbit mAb #15094
Filter:
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25-30 (membrane bound); 17 (soluble) |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #73222.
For a carrier free (BSA and azide free) version of this product see product #73222.
Protocol
Specificity / Sensitivity
CD40 Ligand (D5J9Y) Rabbit mAb recognizes endogenous levels of total membrane bound and soluble CD40 ligand protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CD40 ligand protein.
Background
CD40 ligand (CD40L), also known as CD154, TRAP, and gp39, is the ligand for the TNF receptor family member CD40 (1-6). CD40L is expressed either as a soluble cytokine or as a homotrimeric transmembrane protein. CD40L primarily expressed on the surface of T-cells, but has also been reported in blood platelets, mast cells, basophils, NK cells, and B-cells. It plays an important role in stimulating B-cell cell proliferation and survival and promotes immunoglobulin class switching and secretion of IgE (7). Signals generated by CD40 vary depending on cell type and include activation of MAPK pathways as well as NF-κB (8-11). Mutations within the CD40L gene are associated with X-linked hyper-IgM syndrome characterized by high serum levels of IgM and decreased levels of other isotypes (12). The CD40L/CD40 pathway is an important area of interest in the study of cancer, vascular diseases, and inflammatory disorders (13-15).
- Noelle, R.J. et al. (1992) Proc Natl Acad Sci USA 89, 6550-4.
- Graf, D. et al. (1992) Eur J Immunol 22, 3191-4.
- Armitage, R.J. et al. (1992) Nature 357, 80-2.
- Hollenbaugh, D. et al. (1992) EMBO J 11, 4313-21.
- Gauchat, J.F. et al. (1993) FEBS Lett 315, 259-66.
- Castle, B.E. et al. (1993) J Immunol 151, 1777-88.
- Spriggs, M.K. et al. (1992) J Exp Med 176, 1543-50.
- Li, Y.Y. et al. (1996) J Immunol 157, 1440-7.
- Pearson, L.L. et al. (2001) Int Immunol 13, 273-83.
- Berberich, I. et al. (1996) EMBO J 15, 92-101.
- Aruffo, A. et al. (1993) Cell 72, 291-300.
- Berberich, I. et al. (1994) J Immunol 153, 4357-66.
- Korniluk, A. et al. (2014) Tumour Biol 35, 9447-57.
- Hassan, G.S. et al. (2012) Immunobiology 217, 521-32.
- Zhang, B. et al. (2013) Immunol Lett 153, 58-61.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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