渲染靶标:SSR
Render Timestamp: 2025-03-17T22:47:44.878Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2024-09-30 01:58:40.910
Product last modified at: 2025-02-10T13:45:25.244Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD177 (E1V7N) Rabbit mAb #28740

Filter:
  • WB
  • IHC
Western Blotting Image 1: CD177 (E1V7N) Rabbit mAb
Western blot analysis of extracts from various mouse tissues using CD177 (E1V7N) Rabbit mAb or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 28740

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 105
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #57841.

Protocol

Specificity / Sensitivity

CD177 (E1V7N) Rabbit mAb recognizes endogenous levels of total CD177 protein. Non-specific staining was observed in smooth muscle by immunohistochemistry.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp314 of mouse CD177 protein.

Background

Cluster of differentiation 177 antigen (CD177, also known as human neutrophil alloantigen 2a and NB1), is a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated transmembrane protein expressed primarily on the surface of neutrophils (1,2). Expression of CD177 can be induced by granulocyte-colony-stimulating factor and during pregnancy or bacterial infection (3-5). CD177 mediates adhesion and transendothelial migration of neutrophils via interaction with CD31/PECAM-1 and integrin β2 on endothelial cells (6,7). CD177 also promotes the cell surface expression of proteinase 3 (PR3), which may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (8-10). In association with CD11b/ITGAM, integrin β2, and PR3, CD177 promotes activation of TNF-α primed neutrophils including degranulation and superoxide production (11). CD177 is a polymorphic gene that has been linked to several important clinical diseases, including polycythemia vera, Wegener’s granulomatosis, and immune mediated neonatal neutropenia (12-14).
For Research Use Only. Not For Use In Diagnostic Procedures.
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