R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CD151 (E4I9J) XP® Rabbit mAb #96282
Filter:
- WB
- IHC
Western blot analysis of extracts from various cell lines using CD151 (E4I9J) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, CD151 protein is not expressed in either MOLT-4 or Daudi cells.
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 28 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CD151 (E4I9J) XP® Rabbit mAb recognizes endogenous levels of total CD151 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly138 of human CD151 protein.
Background
CD151 (PETA-3, SFA-1) is a member of the evolutionarily conserved tetraspanin family of multipass glycoproteins (TM4SF), highlighted by four transmembrane domains, two extracellular loops, and N/C-termini that reside within the cytoplasm. Identified as the first member of the tetraspanin family to be implicated in tumorigenesis, research studies have demonstrated that CD151 participates in tumor neovascularization (1), tumor cell cell invasion (2), and cell adhesion (3). Furthermore, a positive correlation exists between CD151 expression levels and poor prognosis for tumors of the lung (4), kidney (5), and prostate (6). CD151 is localized predominantly to the plasma membrane and research studies have demonstrated that CD151 exerts its pro-tumorigenic effects, in part, through the modulation of laminin-binding integrins (7-9) and oncogenic receptor tyrosine kinases, such as c-Met (10,11) and EGFR (12).
- Takeda, Y. et al. (2007) Blood 109, 1524-32.
- Yauch, R.L. et al. (1998) Mol Biol Cell 9, 2751-65.
- Zhang, F. et al. (2011) Blood 118, 4274-84.
- Tokuhara, T. et al. (2001) Clin Cancer Res 7, 4109-14.
- Yoo, S.H. et al. (2011) Histopathology 58, 191-7.
- Ang, J. et al. (2004) Cancer Epidemiol Biomarkers Prev 13, 1717-21.
- Sincock, P.M. et al. (1999) J Cell Sci 112 ( Pt 6), 833-44.
- Winterwood, N.E. et al. (2006) Mol Biol Cell 17, 2707-21.
- Liu, L. et al. (2007) J Biol Chem 282, 31631-42.
- Klosek, S.K. et al. (2009) Biochem Biophys Res Commun 379, 1097-100.
- Franco, M. et al. (2010) J Biol Chem 285, 38756-64.
- Yang, X.H. et al. (2008) Cancer Res 68, 3204-13.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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