R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CD133 (D4W4N) XP® Rabbit mAb (BSA and Azide Free) #69914
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 133 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This product is the carrier free version of product #86781. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #86781
For standard formulation of this product see product #86781
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
CD133 (D4W4N) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD133 protein. The antibody is not sensitive to the glycosylation status of CD133.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the first extracellular domain of human CD133 protein. The epitope has been mapped to a region spanning amino acid residues 257-281, which includes a single N-linked glycosylation site (Asn274).
Background
CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans (8).
- Yin, A.H. et al. (1997) Blood 90, 5002-12.
- Miraglia, S. et al. (1997) Blood 90, 5013-21.
- Handgretinger, R. et al. (2003) Ann N Y Acad Sci 996, 141-51.
- Monzani, E. et al. (2007) Eur J Cancer 43, 935-46.
- O'Brien, C.A. et al. (2007) Nature 445, 106-10.
- Ricci-Vitiani, L. et al. (2007) Nature 445, 111-5.
- Singh, S.K. et al. (2004) Nature 432, 396-401.
- Maw, M.A. et al. (2000) Hum. Mol. Genet. 9, 27-34.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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