R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CD109 (E4I2V) Rabbit mAb (BSA and Azide Free) #34464
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 190 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
This product is the carrier free version of product #24765. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #24765
For standard formulation of this product see product #24765
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
CD109 (E4I2V) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD109 protein. Species cross-reactivity for IHC-P is human only.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val834 of human CD109 protein.
Background
CD109 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein that belongs to the alpha2-macroglobulin family of thioester containing proteins (1). CD109 is associated with TGF-beta receptor I (TbRI) and inhibits TGF-beta signaling (2,3). Cleavage of CD109 at its Furin cleavage site results in the release of its large amino-terminal domain, which then binds to the TGF-beta receptor I to inhibit TGF-beta signaling (4-7). CD109 is expressed on a subset of CD34+ bone marrow cells and mesenchymal stem cells, activated platelets, activated T cells, endothelial cells, and a wide variety of tumors (8-10). Elevated CD109 expression has been considered a diagnostic/prognostic marker for several types of cancers (11-14).
- Lin, M. et al. (2002) Blood 99, 1683-91.
- Finnson, K.W. et al. (2006) FASEB J 20, 1525-7.
- Zhang, J.M. et al. (2015) Biochem Biophys Res Commun 459, 252-8.
- Hagiwara, S. et al. (2010) Oncogene 29, 2181-91.
- Litvinov, I.V. et al. (2011) Exp Dermatol 20, 627-32.
- Zhou, S. et al. (2017) Oncotarget 8, 95632-47.
- Li, C. et al. (2016) Biochem J 473, 537-47.
- Hashimoto, M. et al. (2004) Oncogene 23, 3716-20.
- Sato, T. et al. (2007) Pathol Int 57, 719-24.
- Qi, R. et al. (2018) J Transl Med 16, 88.
- Emori, M. et al. (2015) J Surg Oncol 111, 975-9.
- Jia, W. et al. (2016) Oncotarget 7, 55328-42.
- Yokoyama, M. et al. (2017) Int J Hematol 105, 614-22.
- Chuang, C.H. et al. (2017) Nat Med 23, 291-300.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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