R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CD102/ICAM-2 (D7P2Q) Rabbit mAb #13355
Filter:
- WB
- IP
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45, 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:100 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CD102/ICAM-2 (D7P2Q) Rabbit mAb recognizes endogenous levels of total CD102 (ICAM-2) protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CD102 (ICAM-2) protein.
Background
Intercellular cell adhesion molecule-2 (CD102/ICAM-2) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. Like CD54/ICAM-1, CD102/ICAM-2 is a ligand that binds the leukocyte adhesion molecule LFA-1 (leukocyte function-associated antigen-1), which mediates intercellular interactions between immune cells and other cell types (1).
Expression of CD102/ICAM-2 has been shown to affect angiogenesis (2), cellular radioresistance (3) and anti-tumor immune response (4). Along with CD54/ICAM-1, CD102/ICAM-2 mediates T cell crawling and diapedesis across the blood-brain barrier (5), as well as T cell migration across the bronchial epithelium (6). CD102/ICAM-2 interaction with the actin cytoskeleton through α-actinin has been shown to limit the mobility on neuroblastoma cells (7), and this effect is dependent on glycosylation of CD102/ICAM-2 (8).
Expression of CD102/ICAM-2 has been shown to affect angiogenesis (2), cellular radioresistance (3) and anti-tumor immune response (4). Along with CD54/ICAM-1, CD102/ICAM-2 mediates T cell crawling and diapedesis across the blood-brain barrier (5), as well as T cell migration across the bronchial epithelium (6). CD102/ICAM-2 interaction with the actin cytoskeleton through α-actinin has been shown to limit the mobility on neuroblastoma cells (7), and this effect is dependent on glycosylation of CD102/ICAM-2 (8).
- Staunton, D.E. et al. (1989) Nature 339, 61-4.
- Huang, M.T. et al. (2005) Blood 106, 1636-43.
- Ishigami, T. et al. (2008) Br J Cancer 98, 1357-65.
- Hiraoka, N. et al. (2011) Gastroenterology 140, 310-21.
- Steiner, O. et al. (2010) J Immunol 185, 4846-55.
- Porter, J.C. and Hall, A. (2009) FASEB J 23, 492-502.
- Yoon, K.J. et al. (2008) PLoS One 3, e3629.
- Feduska, J.M. et al. (2013) BMC Cancer 13, 261.
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