R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CCTα (D18B6) Rabbit mAb #6931
Filter:
- WB
- IP
Western blot analysis of extracts from K-562 and HT-1080 cells using CCTα (D18B6) Rabbit mAb.
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CCTα (D18B6) Rabbit mAb recognizes endogenous levels of total CCTα protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CCTα protein.
Background
CTP:phosphocholine cytidylyltransferase (CCT) is a critical enzyme that regulates the CDP-choline pathway for the biosynthesis of phosphatidylcholine. Three distinct CCT isoforms are found in mammals, including CCTα, CCTβ2, and CCTβ3 (1,2). CCTα is the major isoform that is expressed in most tissues (3). CCTα is essential in the synthesis and secretion of surfactant by alveolar epithelial cells and is important in maintaining the phosphatidylcholine level that regulates lipoprotein assembly and secretion in hepatocytes (4,5). CCTα is a major component in membrane biogenesis during cytokine secretion by stimulated macrophages (6). Monoubiquitination of CCTα prevents it from entering the nucleus and leads to its degradation by lysosome (7).
- Jackowski, S. and Fagone, P. (2005) J Biol Chem 280, 853-6.
- Clement, J.M. and Kent, C. (1999) Biochem Biophys Res Commun 257, 643-50.
- Karim, M. et al. (2003) Biochim Biophys Acta 1633, 1-12.
- Tian, Y. et al. (2007) Mol Cell Biol 27, 975-82.
- Jacobs, R.L. et al. (2004) J Biol Chem 279, 47402-10.
- Tian, Y. et al. (2008) J Cell Biol 181, 945-57.
- Chen, B.B. and Mallampalli, R.K. (2009) Mol Cell Biol 29, 3062-75.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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