R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CCL20 (E6J9K) Rabbit mAb #76924
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 9 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CCL20 (E6J9K) Rabbit mAb recognizes endogenous levels of total CCL20 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human CCL20 protein.
Background
Chemokine (C-C motif) ligand 20 (CCL20), also known as macrophage inflammatory protein (MIP)-3α, liver activation regulated chemokine (LARC), and Exodus-1, is a chemokine that mediates leukocyte migration during inflammation and homeostasis (1-3). CCL20 is expressed by T helper (Th) 17 cells (Th17), B cells, natural killer cells, neutrophils, dendritic cells (DC), macrophages, and endothelial cells (4-6). Its receptor, C-C chemokine receptor 6 (CCR6), is expressed by immune cells, such as regulatory CD4+ T cells (Tregs), Th17 cells, CD8+ T cells, and B cells, and drives their migration into CCL20 rich tissues (7). In induced Tregs, CCL20 ligation with its receptor suppresses Treg function via signaling through the AKT/mTOR/STAT3 axis (8). The CCR6-CCL20 axis is being investigated as a therapeutic target for its role in autoimmune disease, viral infection, and cancer progression (7,9).
- Power, C.A. et al. (1997) J Exp Med 186, 825-35.
- Hromas, R. et al. (1997) Blood 89, 3315-22.
- Kadomoto, S. et al. (2020) Int J Mol Sci 21, 5186. doi: 10.3390/ijms21155186.
- Yamazaki, T. et al. (2008) J Immunol 181, 8391-401.
- Scapini, P. et al. (2001) Eur J Immunol 31, 1981-8.
- Nandi, B. et al. (2014) PLoS One 9, e97566.
- Meitei, H.T. et al. (2021) Autoimmun Rev 20, 102846.
- Kulkarni, N. et al. (2018) J Autoimmun 88, 121-130.
- Chen, W. et al. (2020) Adv Exp Med Biol 1231, 53-65.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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