R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Caspase-5 (D3G4W) Rabbit mAb #46680
Filter:
- WB
- IP
Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS #14011 (1 μg/ml, indicated times), using Caspase-5 (D3G4W) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower)
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50, 44, 35 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Caspase-5 (D3G4W) Rabbit mAb recognizes endogenous levels of total caspase-5 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro154 of human caspase-5 protein.
Background
Caspase-5 (Ich-3/ICErelIII/TY) is a member of the caspase family of cysteine proteases that play a key role in the execution of apoptosis and activation of inflammatory cytokines (1-3). Caspase-5 is widely expressed, with highest expression observed in placenta and lung (1). Interferon-γ and LPS regulate expression of caspase-5 (2,4). Members of the caspase-1 subfamily of caspases, which includes caspase-4, -5, and murine caspase-11 and -12, can induce apoptosis when over-expressed and mediate the proteolytic activation of inflammatory cytokines (5). Processing of IL-1β occurs through the activation of an inflammasome complex consisting of caspase-1, caspase-5, Pycard and NALP1 (6). Transcription factor Max, a component of the Myc/Mad/Max network, is cleaved by caspase-5 during Fas-induced apoptosis (7). Several alternative spliced variants of caspase-5 have been identified (8). Frameshift mutations of caspase-5 have been observed in leukemia, lymphoma (9), and colorectal cancers (10).
- Munday, N.A. et al. (1995) J Biol Chem 270, 15870-6.
- Wang, S. et al. (1996) J Biol Chem 271, 20580-7.
- Faucheu, C. et al. (1996) Eur J Biochem 236, 207-13.
- Lin, X.Y. et al. (2000) J Biol Chem 275, 39920-6.
- Martinon, F. and Tschopp, J. (2007) Cell Death Differ 14, 10-22.
- Martinon, F. et al. (2002) Mol Cell 10, 417-26.
- Krippner-Heidenreich, A. et al. (2001) Biochem J 358, 705-15.
- Eckhart, L. et al. (2006) Biochem Biophys Res Commun 348, 682-8.
- Takeuchi, S. et al. (2003) Leuk Res 27, 359-61.
- Trojan, J. et al. (2004) Int J Colorectal Dis 19, 538-44.
Pathways
Explore pathways related to this product.
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