CARD11 Antibody #4440
Filter:
- WB
Western blot analysis of extracts from Raji, Jurkat, Ramos and K562 cells, using CARD11 Antibody.
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CARD11 Antibody detects endogenous levels of total CARD11 protein. Cross-reactivity was not detected with other family members at physiological conditions.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding methionine 362 of human CARD11. Antibodies are purified by protein A and peptide affinity chromatography.
Background
CARD11/Carma1/Bimp3 belongs to the MAGUK (membrane-associated guanylate kinase) family that typically function as molecular scaffolds in the assembly of multiprotein complexes (1,2). MAGUK family members contain an SH3 domain, a PDZ domain and a GuK domain homologous to guanylate kinase. In addition, CARD11 contains an amino-terminal CARD domain (caspase recruitment domain). This domain plays an important role in forming interactions with a number of proteins containing CARD domains that are involved in regulating apoptosis and NF-κB activation. CARD11 is predominately expressed in lymphocytes (1,2) and associates with the CARD domain of Bcl10. When overexpressed, CARD11 leads to the phosphorylation of Bcl10 and activation of NF-κB (1,2). CARD11 is constitutively associated with lipid rafts and is thought to function by recruiting Bcl10 and MALT1 and triggering the phosphorylation of IKKs (3,4). Several studies using the genetic disruption of CARD11 or dominant-negative mutations have demonstrated that it plays a critical role in NF-κB activation and lymphocyte signaling (4-7).
- Bertin, J. et al. (2001) J. Biol. Chem. 276, 11877–11882.
- Gaide, O. et al. (2001) FEBS Lett. 496, 121-127.
- Stilo, R. et al. (2004) J. Biol. Chem. 279, 34323-34331.
- Wang, D. et al. (2002) Nat. Immunol. 3, 830-835.
- Jun, J.E. et al. (2003) Immunity 18, 751-762.
- Hara, H. et al. (2003) Immunity 18, 763-775.
- Gaide, O. et al. (2002) Nat. Immunol. 3, 836-843.
Pathways
Explore pathways related to this product.
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