R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Caldesmon-1 (D5C8D) XP® Rabbit mAb #12503
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70-80 nonmuscle, 120-150 smooth muscle |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Caldesmon-1 (D5C8D) XP® Rabbit mAb recognizes endogenous levels of total caldesmon-1 protein. Based on sequence homology, the antibody is expected to cross-react with both the smooth muscle and nonmuscle isoforms.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human caldesmon-1 protein.
Background
Caldesmon-1 is an actin filament stabilizing protein involved in the regulation of cell contraction. Binding of caldesmon-1 to actin is weakened by phosphorylation and by calmodulin in the presence of calcium. Caldesmon-1 is encoded by a single gene, which is spliced to generate a widely distributed low molecular weight form and a smooth muscle specific high molecular weight form (1,2). Caldesmon-1 is phosphorylated by the cyclin dependent kinase cdc2 and Erk1/2 MAP kinase, both of which prevent the activity of caldesmon-1 (3-5). Phosphorylation of caldesmon-1 by cdc2 is required for passage of cells through mitosis (6). Phosphorylation by Erk1/2 is important in regulating smooth muscle contraction (7). Caldesmon-1 activity may play a role in the formation of podosomes, adhesion complexes associated with the secretion of matrix metalloproteases, invasion, and metastasis (reviewed in 5).
- Hayashi, K. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 12122-12126.
- Humphrey, M.B. et al. (1992) Gene 112, 197-204.
- Yamashiro, S. et al. (1991) Nature 349, 169-172.
- Mak, A.S. et al. (1991) J. Biol. Chem. 266, 6678-6681.
- Hai, C.M. and Gu, Z. (2006) Eur. J. Cell Biol. 85, 305-309.
- Yamashiro, S. et al. (2001) Mol. Biol. Cell 12, 239-250.
- Hedges, J.C. et al. (2000) Am. J. Physiol. Cell Physiol. 278, C718-C7126.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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