Render Target: SSR
Render Timestamp: 2024-11-14T22:38:58.936Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-08-01 15:24:11.992
Product last modified at: 2024-11-04T12:30:49.270Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

C/EBPβ (LAP) Antibody #3087

Filter:
  • WB

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 35 to 38 mouse LAP. 45 to 49 human LAP.
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    C/EBPbeta (LAP) Antibody detects endogenous levels of total C/EBPbeta, the p38 and p36 LAPs, but not the p20 LIP. This antibody does not cross-react with C/EBPalpha, -delta, -gamma, -epsilon or -zeta.

    Species Reactivity:

    Human, Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human C/EBPbeta. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal functions, and inflammatory response (1). Six members of the family have been characterized (C/EBPα, -β, -γ, -δ, -ε, and -ζ) and are distributed in a variety of tissues (1). There are two forms of C/EBPβ, the 38 kDa liver activating protein (LAP) and the 20 kDa liver inhibitory protein (LIP) which may be products of alternative translation. The 38 kDa LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPβ transcriptional activity (2). Phosphorylation of C/EBPβ at distinct sites stimulates its transcriptional activity (3-5). Phosphorylation at serine 105 of rat C/EBPβ, a unique site only present in the rat sequence, seems essential for rat C/EBPβ activation (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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