R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Bub1 (F5L7L) Rabbit mAb #94244
Filter:
- WB
- IP
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:6400 - 1:12800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 - 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Bub1 (F5L7L) Rabbit mAb recognizes endogenous levels of total Bub1 protein. This antibody does not detect Bub1b protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Bub1 protein.
Background
Bub1 is a conserved mitotic serine/threonine-protein kinase that plays a role in activating the spindle assembly checkpoint (SAC), which delays progression into anaphase by inhibiting the anaphase-promoting complex/cyclosome (APC/C) until all chromosome kinetochores are attached to the mitotic spindle. Bub1 also plays a role in chromosome segregation (1,2). Bub1 is overexpressed in multiple human cancers, and its expression is predictive of response to cancer therapies (3). Inhibition of Bub1 sensitizes cancer cells to chemotherapy, radiation, and DNA damage response (DDR) inhibitors (4-6), and reverses taxane resistance in prostate cancer cells (7).
- Manic, G. et al. (2017) Int Rev Cell Mol Biol 328, 105-161.
- Kim, T. and Gartner, A. (2021) Anim Cells Syst (Seoul) 25, 1-10.
- Cicirò, Y. et al. (2024) Sci Rep 14, 4461.
- Wang, W. et al. (2024) Cancers (Basel) 16, 1540.
- Sriramulu, S. et al. (2024) J Exp Clin Cancer Res, 43. doi: 10.1186/s13046-024-03086-9.
- Siemeister, G. et al. (2019) Clin Cancer Res 25, 1404-1414.
- Martinez, M.J. et al. (2023) iScience 26, 107681.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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