R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
BSCL2/Seipin (D3W8C) Rabbit mAb #23846
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
BSCL2/Seipin (D3W8C) Rabbit mAb recognizes endogenous levels of total BSCL2/Seipin protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu383 of human BSCL2 protein.
Background
Seipin/BSCL2 is a homo-oligomeric membrane protein at the endoplasmic reticulum lipid droplet junction (1). Studies have demonstrated that Seipin/BSCL2 plays an important role in adipogenesis, lipid droplet homeostasis, liposis, brain development, and spermatogenesis. However, the exact function of BSCL2/Seipin is still not clear (2-5). BSCL2/Seipin is mutated in several human diseases, including Berardinelli-Seip congenital lipodystrophy, an autosomal recessive disorder characterized by a near absence of adipose tissues, severe insulin resistance, hypertriglyceridemia, hepatic steatosis, and early onset of diabetes (6-8).
- Szymanski, K.M. et al. (2007) Proc Natl Acad Sci U S A 104, 20890-5.
- Liu, L. et al. (2014) Diabetes 63, 2320-31.
- Jiang, M. et al. (2014) Proc Natl Acad Sci U S A 111, 7054-9.
- Ebihara, C. et al. (2015) Hum Mol Genet 24, 4238-49.
- Dollet, L. et al. (2014) Biochimie 96, 166-72.
- Windpassinger, C. et al. (2004) Nat Genet 36, 271-6.
- Boutet, E. et al. (2009) Biochimie 91, 796-803.
- Opri, R. et al. (2016) Seizure 42, 1-6.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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