R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Brn2/POU3F2 (D2C1L) Rabbit mAb #12137
Filter:
- WB
- IP
- IF
- ChIP
- C&R
- C&T
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- ChIP-Chromatin Immunoprecipitation
- C&R-CUT & RUN
- C&T-CUT & Tag
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Frozen) | 1:1600 - 1:3200 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
| CUT&RUN | 1:50 |
| CUT&Tag | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #35173.
For a carrier free (BSA and azide free) version of this product see product #35173.
Protocol
Specificity / Sensitivity
Brn2/POU3F2 (D2C1L) Rabbit mAb recognizes endogenous levels of total Brn2/POU3F2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Brn2/POU3F2 protein.
Background
Brn2/POU3F2 is a POU domain-containing transcription factor involved in neuronal differentiation and activation of the corticotrophin-releasing hormone gene (1,2). In mice, disruption of the Brn2 gene results in loss of specific neuronal lineages in the hypothalamus (3). In addition to its role in mammalian neurogenesis, Brn2 has also been implicated in melanoma tumorigenesis and has been shown in the literature to be overexpressed in human melanoma cells compared to normal melanocytes (4,5). Recent studies also identify Brn2 as a transcription factor playing an important role in keratinocyte differentiation (6). Recent reports demonstrate that overexpression of three transcription factors (Brn2, Ascl1, and Myt1L) can directly convert human fibroblasts into functional neurons under precisely defined conditions (7,8).
- Fujii, H. and Hamada, H. (1993) Neuron 11, 1197-206.
- Schonemann, M.D. et al. (1995) Genes Dev 9, 3122-35.
- Nakai, S. et al. (1995) Genes Dev 9, 3109-21.
- Cook, A.L. et al. (2003) J Invest Dermatol 121, 1150-9.
- Cook, A.L. and Sturm, R.A. (2008) Pigment Cell Melanoma Res 21, 611-26.
- Shi, G. et al. (2010) PLoS One 5, e13216.
- Pfisterer, U. et al. (2011) Proc Natl Acad Sci USA 108, 10343-8.
- Ambasudhan, R. et al. (2011) Cell Stem Cell 9, 113-8.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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