R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
BRD3 (E3D5N) Rabbit mAb #94032
Filter:
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
BRD3 (E3D5N) Rabbit mAb recognizes endogenous levels of total BRD3 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala10 of human BRD3 protein.
Background
Bromodomain-containing protein 3 (BRD3), also known as ORFX, is a member of the bromodomains and extra terminal (BET) family of proteins, which also includes BRD2, BRD4, and BRDT (1-3). BET family proteins contain two tandem bromodomains and an extra terminal (ET) domain, and bind acetyl lysine residues (3). BRD3 is capable of binding acetylated histone H3 Lys14 as well as acetylated histone H4 Lys5 and Lys12 to promote transcription (4). BRD3 plays a role in erythroid development by binding to GATA1 to facilitate its binding to target genes (5). Similar to BRD4, the BRD3 gene can be fused to NUT in NUT midline carcinomas (6). Investigators have found molecular inhibition of BET proteins to be effective in inducing apoptosis in various MLL-fusion driven leukemic cell lines by competing BRD3 and BRD4 from chromatin, leading to reduced expression of BCL2, Myc, and CDK6 (7).
- Belkina, A.C. and Denis, G.V. (2012) Nat Rev Cancer 12, 465-77.
- Voigt, P. and Reinberg, D. (2011) Genome Biol 12, 133.
- Wu, S.Y. and Chiang, C.M. (2007) J Biol Chem 282, 13141-5.
- LeRoy, G. et al. (2008) Mol Cell 30, 51-60.
- Lamonica, J.M. et al. (2011) Proc Natl Acad Sci USA 108, E159-68.
- French, C.A. et al. (2008) Oncogene 27, 2237-42.
- Dawson, M.A. et al. (2011) Nature 478, 529-33.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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