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Product Name

81694 Brachyury (D2Z3J) Rabbit mAb

Filter:

WB

Inquiry Info. # 13165

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Supporting Data

REACTIVITY	H
SENSITIVITY	Transfected Only
MW (kDa)	56
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Brachyury (D5Y9I) Rabbit mAb recognizes transfected levels of total brachyury protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro233 of human brachyury protein.

Background

Brachyury protein, encoded by the T gene, is a transcription factor that is vital for the formation of posterior mesoderm and axial development during vertebrate embryogenesis (1). In the mouse, brachyury is necessary for mesodermal morphogenetic cell movements during gastrulation. Brachyury mutant mice die in utero and display deficient mesoderm formation including an abnormal notochord, missing posterior somites, and a reduced allantois (2). Human brachyury is expressed in the notochord, as well as in chordoma tumors that occur along the spine, making it a good marker for notochord and notochord-derived tumors (3,4). A common polymorphism in the human T gene has also been shown to be associated with development of the multifactorial neural tube defect, spina bifida (5,6).

Database Links

UniProt ID: O15178

Entrez-Gene Id: 6862

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Brachyury (D5Y9I) Rabbit mAb #13165