R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
BORIS (F6R3W) Rabbit mAb #10806
Filter:
- WB
- IP
- IF
- F
- ChIP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 - 1:800 |
| Chromatin IP | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
BORIS (F6R3W) Rabbit mAb recognizes endogenous levels of total BORIS protein. This antibody is predicted to detect isoforms 1, 7, and 8. This antibody detects a band of approximately 70 kDa that is predicted to be a BORIS isoform or cleavage product.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro629 of human BORIS protein.
Background
Brother of the Regulator of Imprinted Sites (BORIS), also known as CCCTC-binding factor like (CTCFL), is a DNA-binding protein that functions as a transcriptional modulator. Similar to CCCTC-binding factor (CTCF), BORIS plays critical roles in chromatin organization and is thought to impact the chromatin landscape by interfering with CTCF-mediated chromatin loops (1,2). BORIS can also act as a transcriptional coactivator by recruiting other activators and chromatin-modifying proteins (3-5). Unlike CTCF, which is expressed uniformly in both healthy and cancerous tissues, BORIS expression is normally restricted to the testis and embryonic stem cells (6,7). However, BORIS is aberrantly expressed in various types of cancer and it is associated with high-risk features that include resistance to treatment (8-11).
- Pugacheva, E.M. et al. (2015) Genome Biol 16, 161.
- Lobanenkov, V.V. and Zentner, G.E. (2018) Nucleus 9, 33-41.
- Kang, Y. et al. (2007) Oncogene 26, 4394-403.
- Jelinic, P. et al. (2006) PLoS Biol 4, e355.
- Nguyen, P. et al. (2008) Mol Cell Biol 28, 6720-9.
- Loukinov, D.I. et al. (2002) Proc Natl Acad Sci U S A 99, 6806-11.
- Monk, M. et al. (2008) Mol Hum Reprod 14, 347-55.
- Klenova, E.M. et al. (2002) Semin Cancer Biol 12, 399-414.
- Martin-Kleiner, I. (2012) Eur J Cancer 48, 929-35.
- Garikapati, K.R. et al. (2017) Biochem Biophys Res Commun 484, 93-99.
- Debruyne, D.N. et al. (2019) Nature 572, 676-680.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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