R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
BOB-1/OBF-1 (E5K1D) Rabbit mAb #43079
Filter:
- WB
- IP
- IHC
- F
Western blot analysis of extracts from various cell lines using BOB-1/OBF-1 (E5K1D) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, BOB-1/OBF-1 protein is not detected in either HDLM-2 or HeLa cells.
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 35 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| IHC Leica Bond | 1:200 - 1:800 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:1600 - 1:6400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #65452.
For a carrier free (BSA and azide free) version of this product see product #65452.
Protocol
Specificity / Sensitivity
BOB-1/OBF-1 (E5K1D) Rabbit mAb recognizes endogenous levels of total BOB-1/OBF-1 protein. Non-specific staining was observed in pancreatic islets by immunohistochemistry.
Species Reactivity:
Human
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro209 of human BOB-1/OBF-1 protein.
Background
B cell Oct binding factor-1 (BOB-1/OBF-1) is a B cell restricted transcriptional coactivator. BOB-1 facilitates transactivation of immunoglobulins and other B cell specific genes through the binding and activation of the transcription factors Oct-1 and Oct-2 (1-4). Research studies have demonstrated that BOB-1 expression is required for antigen-dependent B cell maturation (5-7). In pathological conditions such as classical Hodgkin disease, loss of BOB-1 expression is thought, in part, to contribute to the defect in immunoglobulin gene expression by Hodgkin and Reed Sternberg cells (8,9). In the context of multiple myeloma, overexpression of BOB-1 has been shown to contribute to malignant plasma cell growth, in part, through enhanced transactivation of TNFRSF17/BCMA (10).
- Strubin, M. et al. (1995) Cell 80, 497-506.
- Laumen, H. et al. (2000) Eur J Immunol 30, 458-69.
- Gstaiger, M. et al. (1995) Nature 373, 360-2.
- Brunner, C. and Wirth, T. (2006) Nucleic Acids Res 34, 1807-15.
- Hess, J. et al. (2001) Mol Cell Biol 21, 1531-9.
- Kim, U. et al. (1996) Nature 383, 542-7.
- Schubart, D.B. et al. (1996) Nature 383, 538-42.
- Re, D. et al. (2001) Cancer Res 61, 2080-4.
- Stein, H. et al. (2001) Blood 97, 496-501.
- Zhao, C. et al. (2008) Oncogene 27, 63-75.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
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