R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
BMP6 (Precursor Specific) (D2V5Z) Rabbit mAb #70583
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55-65 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
BMP6 (Precursor Specific) (D2V5Z) Rabbit mAb recognizes endogenous levels of total precursor BMP6 protein. This antibody does not recognize mature BMP6 protein. This antibody also recognizes an unknown protein with a 26 kDa molecular weight.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln173 of human BMP6 protein.
Background
Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs belong to the TGF-β superfamily, playing many diverse functions during development (3). BMPs are synthesized as precursor proteins and then processed by cleavage to release the C-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5, and 8), resulting in the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4).
BMP6 plays a critical role in iron metabolism, ovulation, and aldosterone production (5-7). Recent studies have shown that hypermethylation of the BMP6 gene promoter decreases its expression in several cancer types, which contributes to tumor growth and progression (8-11).
BMP6 plays a critical role in iron metabolism, ovulation, and aldosterone production (5-7). Recent studies have shown that hypermethylation of the BMP6 gene promoter decreases its expression in several cancer types, which contributes to tumor growth and progression (8-11).
- Wang, E.A. et al. (1988) Proc Natl Acad Sci USA 85, 9484-8.
- Wozney, J.M. et al. (1988) Science 242, 1528-34.
- Kawabata, M. et al. (1998) Cytokine Growth Factor Rev 9, 49-61.
- Nohe, A. et al. (2004) Cell Signal 16, 291-9.
- Roth, M.P. and Coppin, H. (2009) Med Sci (Paris) 25, 678-80.
- Inagaki, K. et al. (2006) Endocrinology 147, 2681-9.
- Akiyama, I. et al. (2014) Reprod Sci 21, 772-7.
- Hashida, Y. et al. (2012) Oncol Rep 27, 825-30.
- Liu, G. et al. (2014) Oncol Rep 32, 581-8.
- He, Y. et al. (2014) PLoS One 9, e87994.
- Sangplod, P. et al. (2014) Asian Pac J Cancer Prev 15, 7091-5.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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