BDNF Antibody #47808
Filter:
- WB
Western blot analysis of extracts from 293T cells, untransfected or transfected with human BDNF protein (upper, lanes 1 and 2), or treated with human recombinant Human Brain-Derived Neurotrophic Factor (BDNF) #3897 protein, at the indicated levels, using BDNF Antibody (upper, lanes 3 and 4). Myc-Tag Antibody #2272 was used to show plasmid expression (middle). α-Actinin (D6F6) XP® Rabbit mAb #6487 was used as a loading control (lower).
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Recombinant and Transfected |
| MW (kDa) | Precursor 28 - Mature 12-14 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
BDNF Antibody recognizes human and mouse recombinant BDNF, and exogenously expressed BDNF.
Species Reactivity:
Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid His129 of human BDNF protein.
Background
Neurotrophins are composed of at least four family members, including NGF, BDNF, NT-3, and NT-4, and all are known to influence growth, development, differentiation, and survival of neurons (1). Proneurotrophins bind to p75NTR, but not to the family of Trk receptor tyrosine kinases (Trk), and following maturation, BDNF binds and activates TrkB. Trk receptors, in turn, activate three major signaling pathways: (a) Ras-MAPK signaling, which promotes neuronal differentiation and neurite outgrowth, (b) PI3K/Akt signaling, which promotes survival and growth of neurons, and (c) PLC-γ1-PKC signaling, which promotes synaptic plasticity (2). BDNF is a major regulator of transmission and plasticity at adult synapses. Moreover, the precursor proBDNF and the mature protein mBDNF drive opposite effects on long-term potentiation and long-term depression (3). BDNF has also been implicated in body weight regulation and activity: heterozygous BDNF knockout mice are hyperphagic, obese, and hyperactive (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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