R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Basigin/EMMPRIN (E1S1V) Rabbit mAb (BSA and Azide Free) #95203
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 38-58 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This product is the carrier free version of product #13287. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #13287
For standard formulation of this product see product #13287
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
Basigin/EMMPRIN (E1S1V) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total EMMPRIN protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala254 of human EMMPRIN protein.
Background
Basigin (EMMPRIN, CD147) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily (1). Basigin is a glycosylated protein with four known isoforms, of which isoform 2 is the most abundantly expressed (2). Multiple functions have been ascribed to Basigin; foremost among these is stimulating the secretion of extracellular matrix metalloproteinases by adjacent fibroblasts, a function which has been implicated in promoting tumor progression (2-4). Research studies have shown that Basigin is overexpressed by many tumor cells, and its expression level may correlate with tumor malignancy (5,6). A recent study identified the BASIGIN gene as a regulatory target of Slug, suggesting a role for Basigin in the process of epithelial-mesenchymal transition (7). Basigin has also been identified as a marker for a subset of highly suppressive regulatory T cells (8), and as an obligate receptor for the malarial parasite Plasmodium falciparum on human erythrocytes (9).
- Biswas, C. et al. (1995) Cancer Res 55, 434-9.
- Liao, C.G. et al. (2011) Mol Cell Biol 31, 2591-604.
- Sweeny, L. et al. (2012) Exp Cell Res 318, 1788-98.
- Lescaille, G. et al. (2012) BMC Cancer 12, 115.
- Piao, S. et al. (2012) J Cancer Res Clin Oncol 138, 627-35.
- Bi, X.C. et al. (2011) Clin Invest Med 34, E358.
- Wu, J. et al. (2011) Oncogene 30, 4410-27.
- Solstad, T. et al. (2011) Blood 118, 5141-51.
- Crosnier, C. et al. (2011) Nature 480, 534-7.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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