Render Target: SSR
Render Timestamp: 2024-11-14T22:37:49.316Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-08-01 15:31:14.759
Product last modified at: 2024-11-09T00:15:08.701Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

β-Tubulin Antibody #2146

Filter:
  • WB
  • IHC
  • IF
  • F

    Supporting Data

    REACTIVITY H M R Mk Z B
    SENSITIVITY Endogenous
    MW (kDa) 55
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Z-Zebrafish 
    • B-Bovine 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:75 - 1:300
    Immunofluorescence (Immunocytochemistry) 1:50
    Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    The β-Tubulin Antibody detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, Zebrafish, Bovine

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Xenopus

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human β-tubulin. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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