R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
β-Synuclein (E9R3I) Rabbit mAb #26278
Filter:
- WB
- IP
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing myc-tagged full-length human α-synuclein (hSNCA; +) or myc-tagged full-length human β-synuclein (hSNCB; +), using β-Synuclein (E9R3I) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 18 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
β-Synuclein (E9R3I) Rabbit mAb recognizes endogenous levels of total β-synuclein protein. This antibody does not cross-react with α-synuclein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human β-synuclein protein.
Background
The synuclein family, consisting of α-synuclein, β-synuclein, and γ-synuclein, is a group of small, soluble proteins containing a highly conserved α-helical lipid-binding motif. α-synuclein and β-synuclein are primarily expressed in brain tissue, where they colocalize within presynaptic terminals (1,2). α-synuclein is linked genetically and neuropathologically to Parkinson’s disease and other neurodegenerative diseases, called synucleinopathies, characterized by the presence of intracellular α-synuclein aggregates, the main component of Lewy bodies (3,4). β-synuclein has been shown to inhibit α-synuclein aggregation and ameliorate α-synuclein neurotoxicity (5-7). Studies have shown that β-synuclein levels are elevated in early and advanced Alzheimer’s disease, Creutzfeldt-Jakob disease, and traumatic brain injury, showing strong potential for use as a synaptic damage biomarker (8-9).
- George, J.M. (2002) Genome Biol 3, REVIEWS3002.
- Vigneswara, V. et al. (2013) PLoS One 8, e61442.
- Kim, W.S. et al. (2014) Alzheimers Res Ther 6, 73.
- Stefanis, L. (2012) Cold Spring Harb Perspect Med 2, a009399.
- Hashimoto, M. et al. (2001) Neuron 32, 213-23.
- Brown, J.W. et al. (2016) Sci Rep 6, 36010.
- Fan, Y. et al. (2006) Hum Mol Genet 15, 3002-11.
- Halbgebauer, S. et al. (2021) J Neurol Neurosurg Psychiatry 92, 349-356.
- Halbgebauer, R. et al. (2022) Int J Mol Sci 23, 9639. doi: 10.3390/ijms23179639.
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