R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
AUF1/hnRNP D (D6O4F) Rabbit mAb #12382
Filter:
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 37-48 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
AUF1/hnRNP D (D6O4F) Rabbit mAb recognizes endogenous levels of total AUF1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AUF1 protein.
Background
AU-rich element RNA binding protein 1 (AUF1) is also known as heterogeneous ribonucleoprotein D (hnRNP D). AUF1 binds to the AU rich element (ARE) of target mRNA and regulates mRNA decay (1,2). It has a broad range of target genes including IL-1, IL-2, IL-3, Myc, TNF-α, and cyclin D1 (2). Binding of AUF1 to Myc mRNA also affects translation of Myc (3). Recent studies have provided evidence that AUF1 is also involved in the regulation of transcription. AUF1 binds to the promoters of various genes including complement receptor 2 (4), enkephalin (5), and α-fetoprotein (6). AUF1 also binds to the telomerase catalytic subunit Tert promoter and the G-rich telomeric repeat, thus regulating telomere maintenance and normal aging (7,8). AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (9,10). All AUF1 isoforms shuttle between the nucleus and cytoplasm (11, 12). These isoforms have distinct localization and bind to different target mRNAs that contribute to the diversity of AUF1 function (2).
- Brewer, G. (1991) Mol Cell Biol 11, 2460-6.
- Gratacós, F.M. and Brewer, G. (2010) Wiley Interdiscip Rev RNA 1, 457-73.
- Liao, B. et al. (2007) Nat Struct Mol Biol 14, 511-8.
- Tolnay, M. et al. (2000) Biochem J 348 Pt 1, 151-8.
- Dobi, A. et al. (2006) J Biol Chem 281, 28889-900.
- Jiao, R. et al. (2006) J Cell Biochem 98, 1257-70.
- Eversole, A. and Maizels, N. (2000) Mol Cell Biol 20, 5425-32.
- Pont, A.R. et al. (2012) Mol Cell 47, 5-15.
- Dempsey, L.A. et al. (1998) Genomics 49, 378-84.
- Wagner, B.J. et al. (1998) Genomics 48, 195-202.
- Zhang, W. et al. (1993) Mol Cell Biol 13, 7652-65.
- Sarkar, B. et al. (2003) J Biol Chem 278, 20700-7.
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