R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ATRX (E5X7O) Rabbit mAb (BSA and Azide Free) #67615
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 280 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
This product is the carrier free version of product #10321. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #10321
For standard formulation of this product see product #10321
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
ATRX (E5X7O) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total ATRX protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human ATRX protein.
Background
α-thalassemia/mental retardation X-linked (ATRX) is a transcriptional regulator and helicase that belongs to the SNF2 family of chromatin remodeling proteins (1,2). Together with its binding partner death-associated protein 6 (Daxx), ATRX acts as histone chaperone to deposit histone variant H3.3 at repetitive DNA sequences such as telomeric, pericentric, and ribosomal gene repeats (3-6). ATRX is involved in many nuclear functions that ensure proper sister chromatid cohesion during mitosis and chromosome alignment during meiosis (7,8). The ATRX transcriptional regulator also plays a role in the maintenance of telomere integrity and the regulation of gene expression during mammalian development by influencing DNA methylation patterns at high DNA repeat sequences (9,10). Mutations in the corresponding ATRX gene results in ATR-X syndrome, an X-linked disorder characterized by intellectual disabilities, craniofacial abnormalities, and mild α-thalassemia (11,12). Research studies indicate that the loss of ATRX protein occurs in numerous cancers, including pancreatic neuroendocrine tumors (PanNETs) and pediatric glioblastoma, where telomere maintenance occurs independently of telomerase (13-16).
- Clynes, D. et al. (2013) Trends Biochem Sci 38, 461-6.
- Picketts, D.J. et al. (1996) Hum Mol Genet 5, 1899-907.
- Drané, P. et al. (2010) Genes Dev 24, 1253-65.
- Elsässer, S.J. et al. (2012) Nature 491, 560-5.
- Lewis, P.W. et al. (2010) Proc Natl Acad Sci U S A 107, 14075-80.
- Goldberg, A.D. et al. (2010) Cell 140, 678-91.
- Ritchie, K. et al. (2008) J Cell Biol 180, 315-24.
- De La Fuente, R. et al. (2004) Dev Biol 272, 1-14.
- Wong, L.H. et al. (2010) Genome Res 20, 351-60.
- Gibbons, R.J. et al. (2000) Nat Genet 24, 368-71.
- Gibbons, R.J. et al. (1995) Cell 80, 837-45.
- Gibbons, R.J. et al. (1995) Hum Mol Genet 4 Spec No, 1705-9.
- Heaphy, C.M. et al. (2011) Science 333, 425.
- Lovejoy, C.A. et al. (2012) PLoS Genet 8, e1002772.
- Schwartzentruber, J. et al. (2012) Nature 482, 226-31.
- Jiao, Y. et al. (2011) Science 331, 1199-203.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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