ATP5O (E7F4U) Rabbit mAb #92658

ATP synthase subunit O (ATP5O, OSCP, or ATP5PO) is a component of the mitochondrial complex V, which functions to generate ATP from ADP. ATP synthase is composed of a soluble catalytic core, F₁, and a membrane-spanning proton (H⁺) channel, F_O. ATP5O is part of the peripheral stalk linking the two structural domains. The ATP5O N-terminus directly interacts with subunit F₁, while the C-terminus directly interacts with F_O and is critical for the oligomycin sensitivity of the H⁺ channel (1,2). The expression of ATP5O is elevated in cancer-associated fibroblasts (CAFs) compared to normal fibroblasts in oral squamous cell carcinoma, reflecting the elevated oxidative phosphorylation requirements for ATP generation in these cells (3). Recent studies have shown elevated ATP5O as a biomarker for prostate and gastric cancers (4,5). Conversely, direct binding of claudin-10 (CLDN10) to ATP5O in the outer mitochondrial membrane increases ATP5O expression and acetylation, leading to disrupted mitochondria and reduced metastasis in clear cell renal cell carcinoma (ccRCC) (6).