R Recombinant
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ATF7IP (F8C9I) Rabbit mAb #28299
Filter:
- WB
- IP
Western blot analysis of extracts from various cell lines using ATF7IP (F8C9I) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Low expression of ATF7IP protein in HuH-6 cells is consistent with the predicted expression pattern.
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 190, 220 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Protocol
Specificity / Sensitivity
ATF7IP (F8C9I) Rabbit mAb recognizes endogenous levels of total ATF7IP protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human ATF7IP protein.
Background
Activating transcription factor 7-interacting protein (ATF7IP), also known as MCAF1 or AM, is a repressive chromatin factor that binds to SETDB1, enhancing its ability to catalyze histone H3 lysine 9 trimethylation (1-3). ATF7IP has a SUMO binding domain, which interacts with sumoylated MBD1 to recruit the SETDB1/ATF7IP heterodimer to sites of heterochromatin (4). Loss of ATF7IP results in degradation of SETDB1 protein and a global loss of H3K9me3 (5). ATF7IP can localize to PML bodies in a SUMO-dependent manner, causing reduced histone mRNA and protein and cellular senescence (6). ATF7IP has also been implicated in cancer, where it interacts with SP1 to regulate telomerase. It can also contribute to immune cell function by inhibiting the IL-2 and IL-7R genes (7-9).
- De Graeve, F. et al. (2000) Oncogene 19, 1807-19.
- Wang, H. et al. (2003) Mol Cell 12, 475-87.
- Ichimura, T. et al. (2005) J Biol Chem 280, 13928-35.
- Uchimura, Y. et al. (2006) J Biol Chem 281, 23180-90.
- Timms, R.T. et al. (2016) Cell Rep 17, 653-659.
- Sasai, N. et al. (2013) PLoS One 8, e68478.
- Liu, L. et al. (2009) J Biol Chem 284, 5165-74.
- Sin, J.H. et al. (2019) J Exp Med 216, 2024-2037.
- Sin, J.H. et al. (2022) J Immunol 208, 1155-1169.
限制使用
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