R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ATF-3 (E9J4N) Rabbit mAb #18665
Filter:
- WB
- IP
- IF
- F
- ChIP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 23, 24 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
For optimal ChIP results, use 5 μl of antibody
and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
| Chromatin IP | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ATF-3 (E9J4N) Rabbit mAb recognizes endogenous levels of total ATF-3 protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human ATF-3 protein.
Background
Activating transcription factor 3 (ATF-3) is a basic leucine zipper-type transcription factor belonging to the ATF/cAMP responsive element binding protein family. ATF-3 can form homodimers or heterodimers with other family members and depending on the cell types and promoter context, it can suppress or activate the transcription of its target genes (1,2). Expression of ATF-3 is induced by a variety of factors including cytokines, genotoxic agents, and physiological stress. For example, both metformin and high-density lipoprotein (HDL) can induce ATF-3 expression in macrophages (1,3-5). Research studies also indicate that ATF-3 can function as an oncogene or a tumor suppressor depending on the context (6,7).
- Hai, T. et al. (1999) Gene Expr 7, 321-35.
- Hai, T. and Hartman, M.G. (2001) Gene 273, 1-11.
- Hai, T. et al. (2010) Gene Expr 15, 1-11.
- De Nardo, D. et al. (2014) Nat Immunol 15, 152-60.
- Kim, J. et al. (2014) J Biol Chem 289, 23246-55.
- Yin, X. et al. (2008) Oncogene 27, 2118-27.
- Wang, Z. et al. (2014) Oncogene Advanced online publication, doi:10.1038/onc.2014.426.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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