R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ARID4A/RbBP1 (E2T6V) Rabbit mAb #38328
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 205 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ARID4A/RbBP1 (E2T6V) Rabbit mAb recognizes endogenous levels of total ARID4A/RbBP1 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly813 of human ARID4A/RbBP1 protein.
Background
ARID4A/RbBP1 was originally identified as an RB binding protein, which could repress E2F-mediated transcription through the recruitment of the mSIN3A complex (1-3). Like other AT-rich interacting domain (ARID) containing proteins, ARID4A/RbBP1 functions as a tumor suppressor, and is frequently mutated in colorectal cancer (4-6). ARID4A/RbBP1 and ARID4B/RbBP1L1 are mainly expressed in Sertoli cells in the testes, where they form a complex and serve as coactivators for androgen receptors to regulate spermatogenesis (7).
- Defeo-Jones, D. et al. (1991) Nature 352, 251-4.
- Lai, A. et al. (1999) Oncogene 18, 2091-100.
- Lai, A. et al. (1999) Mol Cell Biol 19, 6632-41.
- Hurst, D.R. et al. (2008) J Biol Chem 283, 7438-44.
- Wu, M.Y. et al. (2008) J Natl Cancer Inst 100, 1247-59.
- Cajuso, T. et al. (2014) Int J Cancer 135, 611-23.
- Wu, R.C. et al. (2013) Proc Natl Acad Sci U S A 110, 4616-21.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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