R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ARHGAP29 (F6O5B) Rabbit mAb #37001
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 142 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ARHGAP29 (F6O5B) Rabbit mAb recognizes endogenous levels of total ARHGAP29 protein. By western blot, this antibody detects a band of unknown origin at approximately 10 kDa.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly561 of human ARHGAP29 protein.
Background
Rho GTPase-activating protein 29 (ARHGAP29), also known as PARG1, suppresses Rho family GTPase signaling by promoting GTP hydrolysis and the inactive (GDP-bound) state. Transcription of ARHGAP29 is positively regulated by Yes-associated protein (YAP), destabilizing the actin cytoskeleton and promoting cell migration (1). Upregulated expression of ARHGAP29 is associated with invasion and proliferation in human prostate, breast, and kidney cancer cells, and may serve as a prognostic indicator in several human cancers (1-4). Mutation of the ARHGAP29 gene is associated with nonsyndromic cleft lip and/or palate (NSCL/P) in humans (5,6).
- Qiao, Y. et al. (2017) Cell Rep 19, 1495-1502.
- Shimizu, K. et al. (2020) Oncol Rep 44, 2735-2745.
- Kolb, K. et al. (2020) Cells 9, 2616. doi: 10.3390/cells9122616.
- Miyazaki, J. et al. (2017) Transl Oncol 10, 142-152.
- Leslie, E.J. et al. (2012) Birth Defects Res A Clin Mol Teratol 94, 934-42.
- Liu, H. et al. (2017) Birth Defects Res 109, 27-37.
限制使用
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专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
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