R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
APEX2 (E5A2Z) Rabbit mAb #74728
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
APEX2 (E5A2Z) Rabbit mAb recognizes endogenous levels of total APEX2 protein. This antibody cross-reacts with an aproximately 100 kDa band of unknown origin by western blot.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu393 of human APEX2 protein.
Background
DNA-(apurinic or apyrimidinic site) endonuclease 2 (APEX2/APE2) is an endodeoxyribonuclease that functions in the DNA base excision repair (BER) pathway to repair DNA lesions induced by oxidative and alkylating agents. APEX2 acts in ATR-dependent DNA damage checkpoint activation in response to oxidative stress (1). The APEX2 gene is synthetic lethal with BRCA1 and BRCA2 genes, which are required for homologous recombination (HR) repair of double-stranded DNA breaks. This is due to the ability of APEX2 to reverse single-stranded DNA breaks with 3’ end blocks. Because BRCA-deficient cancer cells are sensitive to 3’ blocks, APEX2 is a potential therapeutic target for HR-deficient cancers (2,3).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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