AP-2γ Antibody #2320
Filter:
- WB
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 49 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
AP-2γ Antibody detects endogenous levels of total AP-2γ protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human AP-2γ. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Activator protein 2γ (AP-2γ) is a member of the developmentally-regulated transcription factor activator protein 2 family (1). Overexpression of p53 in human breast cancer cells increases AP-2γ mRNA and protein (2). In addition, p53 binds to the AP-2γ promoter, suggesting that AP-2γ is a target of p53. AP-2γ therefore may contribute to p53-mediated growth inhibition (2). Studies also found that AP-2γ increases p21 mRNA and protein levels and induces cell-cycle arrest in G1 phase (3). Overexpression of AP-2γ inhibits the growth of human breast cancer cells (3). Taken together, these findings suggest that AP-2γ functions as a tumor suppressor (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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