R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ANP32A (D7Z5U) Rabbit mAb #15491
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 28 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:3000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ANP32A (D7Z5U) Rabbit mAb recognizes endogenous levels of total ANP32A protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino terminus of human ANP32A protein.
Background
ANP32A is the founding member of the acidic nuclear phosphoprotein 32 kDa (ANP32) family, which share two highly conserved regions: the N-terminal leucine-rich repeats (LRRs) sequence and the C-terminal acidic tail (1). ANP32A was originally purified as a potent, heat-stable protein phosphatase 2A (PP2A)-specific inhibitor, and subsequent studies suggested that it binds directly to the catalytic subunit of PP2A (2,3). ANP32A is a key component of acetyltransferase inhibitor complex that regulates chromatin remodeling and transcription (4,5). ANP32A also forms a multiunit complex with HuR that regulates RNA transport and stability (6). In addition, ANP32A is reported to play roles in apoptosis, neural differentiation and pathogenesis (7-10).
- Matilla, A. and Radrizzani, M. (2005) Cerebellum 4, 7-18.
- Li, M. et al. (1995) Biochemistry 34, 1988-96.
- Li, M. et al. (1996) Biochemistry 35, 6998-7002.
- Seo, S.B. et al. (2002) J Biol Chem 277, 14005-10.
- Schneider, R. et al. (2004) J Biol Chem 279, 23859-62.
- Brennan, C.M. et al. (2000) J Cell Biol 151, 1-14.
- Jiang, X. et al. (2003) Science 299, 223-6.
- Chen, S. et al. (2008) J Biol Chem 283, 10513-21.
- Kular, R.K. et al. (2009) J Biol Chem 284, 7783-92.
- Cvetanovic, M. et al. (2012) Neurobiol Dis 48, 526-32.
限制使用
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