渲染靶标:SSR
Render Timestamp: 2025-04-02T00:04:06.308Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:10:14.581
Product last modified at: 2025-03-19T13:15:10.666Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Androgen Receptor (D6F11) XP® Rabbit mAb #5153

Filter:
  • WB
  • IP
  • IHC
  • IF
  • F
  • ChIP
  • C&R
Western Blotting Image 1: Androgen Receptor (D6F11) XP® Rabbit mAb
Western blot analysis of extracts from LNCaP (AR+), MCF7 (AR+), PC-3 (AR-), and DU 145 (AR-) cells using Androgen Receptor (D6F11) XP® Rabbit mAb (upper) and β-Actin Antibody #4967 (lower).

To Purchase # 5153

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 110
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
  • ChIP-Chromatin Immunoprecipitation 
  • C&R-CUT & RUN 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

The CUT&RUN dilution was determined using the CUT&RUN Assay Kit #86652.
Application Dilution
Western Blotting 1:2000
Simple Western™ 1:50 - 1:250
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:200 - 1:800
Immunofluorescence (Immunocytochemistry) 1:600 - 1:1200
Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:800
Chromatin IP 1:100
Chromatin IP-seq 1:100
CUT&RUN 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #23791.

Protocol

Specificity / Sensitivity

Androgen Receptor (D6F11) XP® Rabbit mAb detects endogenous levels of total androgen receptor protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to residues near the amino terminal region of human androgen receptor protein.

Background

Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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XP is a registered trademark of Cell Signaling Technology, Inc.
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