ALPP (3E5C7) Mouse mAb #8681
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| Source/Isotype | Mouse IgG2b |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ALPP (3E5C7) Mouse mAb recognizes endogenous levels of total ALPP protein. This antibody may cross-react with a protein of unknown origin at ~140 kDa in some cell lines.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human ALPP protein.
Background
Mammalian alkaline phosphatases (ALPs) are highly conserved zinc-containing allosteric enzymes that hydrolyze and transphosphorylate a wide range of compounds (1). There are four known human alkaline phosphatase isozymes: ALPL (tissue-nonspecific; bone/liver/kidney), ALPP (placental), ALPPL2 (germ cell), and ALPI (intestinal) (2). Placental alkaline phosphatase (ALPP) is bound to the plasma membrane via a glycosyl-phosphatidylinositol (GPI) anchor (3). It is expressed primarily in the placenta (4) and may be involved in transplacental IgG transport (5). ALPP has been found to be overexpressed on the surface of several different types of solid tumor cells (6), and elevated serum concentrations of ALPP and ALPP-like enzymes has been found to be associated with ovarian, cervical, and testicular cancer (7).
- Hoylaerts, M.F. et al. (1997) J Biol Chem 272, 22781-7.
- Millán, J.L. (2006) Purinergic Signal 2, 335-41.
- Low, M.G. and Saltiel, A.R. (1988) Science 239, 268-75.
- Beckman, G. et al. Hum Hered 45, 1-5.
- Fishman, L. et al. (1976) Cancer Res 36, 2268-73.
- Ind, T. et al. (1997) Br J Obstet Gynaecol 104, 1024-9.
- Koshida, K. et al. (1996) Br J Urol 77, 138-42.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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