R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ALOX12 (E3O9P) Rabbit mAb #49874
Filter:
- WB
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 76 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ALOX12 (E309P) Rabbit mAb recognizes endogenous levels of total ALOX12 protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly608 of human ALOX12 protein.
Background
Lipoxygenases catalyze the oxidation of polyunsaturated fatty acids and have a variety of physiological functions as well as roles in disease (1). ALOX12 (arachidonic acid 12-lipoxygenase) is an important lipoxygenase that specifically converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid (12- HpETE), which is then reduced by glutathione peroxidase to 12-hydroxyeicosatetraenoic acid (12-HETE). ALOX12 plays a role in regulating platelet aggregation, cell migration, and proliferation and is involved in diseases, such as thrombosis, atherosclerosis, diabetes, neurodegeneration, inflammatory disease, and cancer (2). ALOX12 is highly expressed in nonalcoholic steatohepatitis (NASH) and can regulate disease progression by interacting with acetyl-CoA carboxylase 1 (ACC1) (3,4). ALOX12 is also upregulated during hepatic ischemia leading to inflammation and cell death (5). ALOX12 inhibition also abrogates p53-mediated ferroptosis and tumor suppression but does not affect other ferroptosis pathways such as that driven by GPX4 (6).
- Mashima, R. and Okuyama, T. (2015) Redox Biol 6, 297-310.
- Zheng, Z. et al. (2020) Biomed Pharmacother 129, 110354.
- Zhang, X.J. et al. (2021) Sci Transl Med 13, eabg8116.
- Zhang, X.J. et al. (2021) Sci Transl Med 13, eabg8117.
- Zhang, X.J. et al. (2018) Nat Med 24, 73-83.
- Chu, B. et al. (2019) Nat Cell Biol 21, 579-591.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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