R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Alix (E4T7U) Rabbit mAb #18269
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90-100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Alix (E4T7U) Rabbit mAb recognizes endogenous levels of total Alix protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg475 of human Alix protein.
Background
Alix, a phylogenetically conserved cytosolic scaffold protein, contains an N-terminal Bro1 domain, a coiled-coil region and a C-terminal proline-rich domain (1,2). Originally identified as an ALG-2 (apoptosis-linked gene 2)-interacting protein involved in programmed cell death (3,4), Alix also regulates many other cellular processes, such as endocytic membrane trafficking and cell adhesion through interactions with ESCRT (endosomal sorting complex required for transport) proteins, endophilins, and CIN85 (Cbl-interacting protein of 85 kDa) (5,6).
Alix has also been shown to be involved in the biogenesis of exosomes, small secreted vesicles that contribute to cell signaling (7, 8). In addition, Alix interacts with Atg12-Atg3 to promote autophagy and endosomal fusion (9)
Alix has also been shown to be involved in the biogenesis of exosomes, small secreted vesicles that contribute to cell signaling (7, 8). In addition, Alix interacts with Atg12-Atg3 to promote autophagy and endosomal fusion (9)
- Mattei, S. et al. (2006) Eur. J. Cell Biol. 85, 925-936.
- Odorizzi, G. (2006) J. Cell Sci. 119, 3025-3032.
- Vito, P. et al. (1999) J. Biol. Chem. 274, 1533-1540.
- Missotten, M. et al. (1999) Cell Death Differ. 6, 124-129.
- Katoh, K. et al. (2003) J. Biol. Chem. 278, 39104-39113.
- Sadoul, R. (2006) Biol. Cell 98, 69-77.
- Baietti, M.F. et al. (2012) Nat Cell Biol 14, 677-85.
- Chatellard-Causse, C. et al. (2002) J Biol Chem 277, 29108-15.
- Murrow, L. et al. (2015) Nat Cell Biol 17, 300-10.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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