Alix (3A9) Mouse mAb #2171
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 95 |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Alix (3A9) Mouse mAb detects endogenous levels of total Alix protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with full-length recombinant human Alix protein.
Background
Alix, a phylogenetically conserved cytosolic scaffold protein, contains an N-terminal Bro1 domain, a coiled-coil region and a C-terminal proline-rich domain (1,2). Originally identified as an ALG-2 (apoptosis-linked gene 2)-interacting protein involved in programmed cell death (3,4), Alix also regulates many other cellular processes, such as endocytic membrane trafficking and cell adhesion through interactions with ESCRT (endosomal sorting complex required for transport) proteins, endophilins, and CIN85 (Cbl-interacting protein of 85 kDa) (5,6).
- Mattei, S. et al. (2006) Eur. J. Cell Biol. 85, 925-936.
- Odorizzi, G. (2006) J. Cell Sci. 119, 3025-3032.
- Vito, P. et al. (1999) J. Biol. Chem. 274, 1533-1540.
- Missotten, M. et al. (1999) Cell Death Differ. 6, 124-129.
- Katoh, K. et al. (2003) J. Biol. Chem. 278, 39104-39113.
- Sadoul, R. (2006) Biol. Cell 98, 69-77.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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