ALFA-Tag (E1R1A) Mouse mAb #54963
Filter:
- WB
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 kappa |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ALFA-Tag (E1R1A) Mouse mAb detects exogenously expressed proteins containing the ALFA epitope tag. The antibody recognizes the ALFA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. ALFA-Tag (E1R1A) Mouse mAb detects exogenously expressed ALFA-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody may detect several non-specific bands of unknown origin in rat cell lines.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the ALFA epitope tag.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
The ALFA-tag is a highly versatile epitope tag made from a hydrophilic, 15 amino acid sequence with a high propensity to form a small, stable α-helix structure (1).
The ALFA-tag is a highly versatile epitope tag made from a hydrophilic, 15 amino acid sequence with a high propensity to form a small, stable α-helix structure (1).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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