R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Aldolase C (E7I4X) Rabbit mAb #81944
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 41 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Aldolase C (E7I4X) Rabbit mAb recognizes endogenous levels of total fructose bisphosphate aldolase C protein. This antibody does not cross-react with fructose bisphosphate aldolase A or fructose bisphosphate aldolase B proteins. This antibody detects a non-specific band of unknown origin at 75 kDa in mouse lung tissue extracts.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn90 of human aldolase C protein.
Background
Aldolase (fructose bisphosphate aldolase), a glycolytic enzyme, catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde. This ubiquitous enzyme is present as three different isozymes: aldolase A, aldolase B, and aldolase C. Research studies suggest that aldolase A expression potentially differentiates between nonneoplastic liver diseases and hepatocarcinoma (1). Furthermore, investigators have shown that changes in aldolase B gene expression levels have been observed in certain patients with this primary tumor (2,3).
Aldolase C is primarily expressed in the central nervous system (4). Studies have shown increased detection of serum aldolase C following traumatic brain injury (TBI) and mild TBI, showing potential for aldolase C as a biomarker for TBI (5,6).
Aldolase C is primarily expressed in the central nervous system (4). Studies have shown increased detection of serum aldolase C following traumatic brain injury (TBI) and mild TBI, showing potential for aldolase C as a biomarker for TBI (5,6).
- Castaldo, G. et al. (2000) Clin. Chem. 46, 901-906.
- Kinoshita, M. and Miyata, M. (2002) Hepatology 36, 433-438.
- Song, H. et al. (2004) World J. Gastroenterol 10, 509-513.
- Fujita, H. et al. (2014) PLoS One 9, e86679.
- Thelin, E.P. et al. (2018) Behav Brain Res 340, 71-80.
- Haddadi, K. et al. (2022) Iran J Med Sci 47, 33-39.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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