R Recombinant
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ALDH1A2 (E6O6Q) Rabbit mAb #55481
Filter:
- WB
- IF
- F
Western blot analysis of extracts from various cell lines using ALDH1A2 (E6O6Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Expression levels of ALDH1A2 among cell lines are consistent with expectations based on publicly available bioinformatic databases.
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ALDH1A2 (E6O6Q) Rabbit mAb recognizes endogenous levels of total ALDH1A2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro9 of human ALDH1A2 protein.
Background
The aldehyde dehydrogenase family is a large group of enzymes that catalyze the oxidization of aldehydes into carboxylic acids (1). Aldehyde Dehydrogenase 1A2 (ALDH1A2, RALHD2) is among a group of aldehyde dehydrogenases that catalyze the metabolism of retinaldehyde into retinoic acid (RA), which plays a critically important signaling role in animal development (2). Research studies have shown that ALDH1A2 also plays a role postnatally in modulating the effects of RA signaling on immune cell function (3-5). In one example using a genetic mouse model, it was shown that ALDH1A2-dependent RA signaling was a downstream mediator of NOTCH-dependent T cell differentiation (6).
- Jackson, B. et al. (2011) Hum Genomics 5, 283-303.
- Means, A.L. and Gudas, L.J. (1995) Annu Rev Biochem 64, 201-33.
- Dalmas, E. et al. (2017) Immunity 47, 928-942.e7.
- Shiokawa, A. et al. (2017) Immunology 152, 52-64.
- Yokota-Nakatsuma, A. et al. (2016) Sci Rep 6, 37914.
- Zaman, T.S. et al. (2017) J Immunol 199, 1989-97.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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