R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb #9030
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 92 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb recognizes endogenous levels of β-catenin protein only when acetylated at Lys49.
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys49 of human β-catenin protein.
Background
β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
Lys49 lies in a region that contains several Ser/Thr residues whose phosphorylation status regulates the stability of β-catenin. Research studies have shown that Lys49 is one of few residues frequently mutated in thyroid anaplastic carcinoma (9). CREB-binding protein (CBP) binds and acetylates β-catenin at Lys49 (10,11).
Lys49 lies in a region that contains several Ser/Thr residues whose phosphorylation status regulates the stability of β-catenin. Research studies have shown that Lys49 is one of few residues frequently mutated in thyroid anaplastic carcinoma (9). CREB-binding protein (CBP) binds and acetylates β-catenin at Lys49 (10,11).
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
- Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
- Amit, S. et al. (2002) Genes Dev 16, 1066-76.
- Liu, C. et al. (2002) Cell 108, 837-47.
- Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
- Yost, C. et al. (1996) Genes Dev 10, 1443-54.
- Morin, P.J. et al. (1997) Science 275, 1787-90.
- Polakis, P. (2000) Genes Dev 14, 1837-51.
- Takemaru, K.I. and Moon, R.T. (2000) J Cell Biol 149, 249-54.
- Wolf, D. et al. (2002) J Biol Chem 277, 25562-7.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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