Acetyl-α-Tubulin (Lys40) Matched Antibody Pair #53362
Filter:
- ELISA
Data using Acetyl-α-Tubulin (Lys40) Matched Antibody Pair #53362 are shown. The two antibodies function as a capture-target-detection sandwich (#76031 and #77780, respectively) to detect endogenous protein expression in positive and negative controls.
Supporting Data
| REACTIVITY | H M R |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Product Includes
- Related Products
| Product Includes | Quantity | Isotype |
|---|---|---|
| α-Tubulin (DM1A) Mouse mAb (BSA and Azide Free) #76031 | 100 µg | Mouse IgG1 |
| Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (BSA and Azide Free) #77780 | 100 µg | Rabbit IgG |
Product Information
Product Usage Information
Matched Antibody Pairs consist of capture and detection antibodies that bind to non-overlapping epitopes. For specific identification of the capture and detection antibodies in this pair, please refer to the data figure caption. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
Storage
Store at -20ºC. This product will freeze at -20ºC so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Product Description
The Acetyl-α-Tubulin (Lys40) Matched Antibody Pair is ideal for use with immunoassay technologies and high-throughput ELISA platforms requiring antibody pairs with specialized or custom antibody labeling. Labels include fluorophores, lanthanides, biotin, and beads. Platforms requiring conjugated Matched Antibody Pairs include MSD, Quanterix Simoa, Alpha Technology (AlphaScreen, AlphaLISA, LANCE, HTRF), and Luminex.
Learn how Matched Antibody Pairs move your projects forward, faster at cst-science.com/matched-antibody-pairs.
Learn how Matched Antibody Pairs move your projects forward, faster at cst-science.com/matched-antibody-pairs.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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