Abi3 Antibody #23060
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55, 65 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Abi3 Antibody recognizes endogenous levels of total Abi3 protein. This antibody recognizes a band at ~100 kDa of unknown origin.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu182 of human Abi3 protein. Antibodies are purified by peptide affinity chromatography.
Background
Abi1, Abi2, and Abi3 are members of the Abl1 interactor family (Abi), which function as adaptor signaling molecules downstream of the receptor tyrosine kinase Abl1 (1-3). In addition to binding to Abl1, all Abi proteins contain a Src homology 3 (SH3) domain and interact with the WAVE complex, an actin regulator involved in membrane ruffling and lamellipodia formation (4,5). As cytoskeletal adaptor proteins, Abi proteins are likely to regulate actin cytoskeletal remodeling. Interestingly, ABI3, the gene encoding Abi3, is genetically linked to Alzheimer’s disease (AD). Abi3 may contribute to microglia-mediated AD progression by regulating microglial morphology (6,7).
- Shi, Y. et al. (1995) Genes Dev 9, 2583-97.
- Dai, Z. and Pendergast, A.M. (1995) Genes Dev 9, 2569-82.
- Miyazaki, K. et al. (2000) Biochim Biophys Acta 1493, 237-41.
- Hirao, N. et al. (2006) FEBS Lett 580, 6464-70.
- Kotula, L. (2012) FEBS Lett 586, 2790-4.
- Conway, O.J. et al. (2018) Mol Neurodegener 13, 53.
- Sims, R. et al. (2017) Nat Genet 49, 1373-1384.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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